Avocado fruit maturation and ripening: dynamics of aliphatic acetogenins and lipidomic profiles from mesocarp, idioblasts and seed

Hdl Handle:
http://hdl.handle.net/11285/627923
Title:
Avocado fruit maturation and ripening: dynamics of aliphatic acetogenins and lipidomic profiles from mesocarp, idioblasts and seed
Authors:
Rodríguez López, Carlos E; Hernández Brenes, Carmen; Treviño, Víctor; Díaz de la Garza, Rocío I
Issue Date:
2017-09-29
Publisher:
BioMed Central
Discipline:
Ciencias / Sciences
Abstract:

Abstract Background Avocado fruit contains aliphatic acetogenins (oft-acetylated, odd-chain fatty alcohols) with promising bioactivities for both medical and food industries. However, we have scarce knowledge about their metabolism. The present work aimed to study changes in acetogenin profiles from mesocarp, lipid-containing idioblasts, and seeds from ‘Hass’ cultivar during fruit development, germination, and three harvesting years. An untargeted LC-MS based lipidomic analysis was also conducted to profile the lipidome of avocado fruit in each tissue. Results The targeted analysis showed that acetogenin profiles and contents remained unchanged in avocado mesocarp during maturation and postharvest ripening, germination, and different harvesting years. However, a shift in the acetogenin profile distribution, accompanied with a sharp increase in concentration, was observed in seed during early maturation. Untargeted lipidomics showed that this shift was accompanied with remodeling of glycerolipids: TAGs and DAGs decreased during fruit growing in seed. Remarkably, the majority of the lipidome in mature seed was composed by acetogenins; we suggest that this tissue is able to synthesize them independently from mesocarp. On the other hand, lipid-containing idioblasts accumulated almost the entire acetogenin pool measured in the whole mesocarp, while only having 4% of the total fatty acids. The lipidome of this cell type changed the most when the fruit was ripening after harvesting, TAGs decreased while odd-chain DAGs increased. Notably, idioblast lipidome was more diverse than that from mesocarp. Conclusions Evidence shown here suggests that idioblasts are the main site of acetogenin biosynthesis in avocado mesocarp. This work unveiled the prevalence of aliphatic acetogenins in the avocado fruit lipidome and evidenced TAGs as initial donors of the acetogenin backbones in its biosynthesis. It also sets evidence for acetogenins being included in future works aimed at characterizing the avocado seed, as they are a main component of their lipidome.

Keywords:
Avocado fruit; Lauraceous Acetogenins; Aliphatic Acetogenins; Idioblasts; Ripening; Untargeted Lipidomics; TAG catabolism
Additional Links:
https://www.ncbi.nlm.nih.gov/pubmed/28969589
Type:
Artículo / Article
Appears in Collections:
Artículos de Revistas

Full metadata record

DC FieldValue Language
dc.contributor.authorRodríguez López, Carlos Een
dc.contributor.authorHernández Brenes, Carmenen
dc.contributor.authorTreviño, Víctoren
dc.contributor.authorDíaz de la Garza, Rocío Ien
dc.date.accessioned2017-10-08T04:46:29Z-
dc.date.available2017-10-08T04:46:29Z-
dc.date.issued2017-09-29-
dc.identifier.issn1471-2229-
dc.identifier.doihttp://dx.doi.org/10.1186/s12870-017-1103-6-
dc.identifier.urihttp://hdl.handle.net/11285/627923-
dc.description.abstract<p>Abstract Background Avocado fruit contains aliphatic acetogenins (oft-acetylated, odd-chain fatty alcohols) with promising bioactivities for both medical and food industries. However, we have scarce knowledge about their metabolism. The present work aimed to study changes in acetogenin profiles from mesocarp, lipid-containing idioblasts, and seeds from ‘Hass’ cultivar during fruit development, germination, and three harvesting years. An untargeted LC-MS based lipidomic analysis was also conducted to profile the lipidome of avocado fruit in each tissue. Results The targeted analysis showed that acetogenin profiles and contents remained unchanged in avocado mesocarp during maturation and postharvest ripening, germination, and different harvesting years. However, a shift in the acetogenin profile distribution, accompanied with a sharp increase in concentration, was observed in seed during early maturation. Untargeted lipidomics showed that this shift was accompanied with remodeling of glycerolipids: TAGs and DAGs decreased during fruit growing in seed. Remarkably, the majority of the lipidome in mature seed was composed by acetogenins; we suggest that this tissue is able to synthesize them independently from mesocarp. On the other hand, lipid-containing idioblasts accumulated almost the entire acetogenin pool measured in the whole mesocarp, while only having 4% of the total fatty acids. The lipidome of this cell type changed the most when the fruit was ripening after harvesting, TAGs decreased while odd-chain DAGs increased. Notably, idioblast lipidome was more diverse than that from mesocarp. Conclusions Evidence shown here suggests that idioblasts are the main site of acetogenin biosynthesis in avocado mesocarp. This work unveiled the prevalence of aliphatic acetogenins in the avocado fruit lipidome and evidenced TAGs as initial donors of the acetogenin backbones in its biosynthesis. It also sets evidence for acetogenins being included in future works aimed at characterizing the avocado seed, as they are a main component of their lipidome.</p>en
dc.language.isoengen
dc.publisherBioMed Centralen
dc.relation.urlhttps://www.ncbi.nlm.nih.gov/pubmed/28969589en
dc.rightsOpen Accessen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/-
dc.titleAvocado fruit maturation and ripening: dynamics of aliphatic acetogenins and lipidomic profiles from mesocarp, idioblasts and seeden
dc.typeArtículo / Articleen
dc.contributor.departmentTecnologico de Monterreyen
dc.language.rfc3066en-
dc.rights.holderThe Author(s).-
dc.date.updated2017-10-08T03:29:26Z-
dc.subject.keywordAvocado fruiten
dc.subject.keywordLauraceous Acetogeninsen
dc.subject.keywordAliphatic Acetogeninsen
dc.subject.keywordIdioblastsen
dc.subject.keywordRipeningen
dc.subject.keywordUntargeted Lipidomicsen
dc.subject.keywordTAG catabolismen
dc.subject.disciplineCiencias / Sciencesen
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