Studies on the growth of magnetospirillum gryphiswaldense under several conditions and its influence on the production of magnetosomes

Hdl Handle:
http://hdl.handle.net/11285/622540
Title:
Studies on the growth of magnetospirillum gryphiswaldense under several conditions and its influence on the production of magnetosomes
Issue Date:
2017-05-12
Abstract:

In recent years, Magnetospirillum gryphiswaldense has called attention because it is a bacteria capable of producing magnetic nanoparticles (magnetosomes) that can be used in a variety of applications, such as directed therapies for drug delivery. The production of magnetosomes has not reach threshole concentration to ensure feasible for industrial applications. Thus, it is of high importance to increase the magnetosome production. This research evaluated the effect of stirring rate (100, 200, 400 and 600 rpm) and hydrodinamic conditions based on Reynolds number for the grwoth and production of M. gryphiswaldense. Overall, the better results were obtained at 200 rpm, with correspond to higher biomass and oxygen transfer coeficient (KLA). Optimal impeler stirring rate was selected to grow bacteria, followed by an anaerobic stage with feeding, to induce the production of magnetosomes. The best magnetosome yield obtained was 18.79 mg/mL*day in a fed-batch culture, 11.78 in continuous culture and 1.4 in batch culture. Their respective specific growth (μ) and generation time (min) were 0.083 and 8.31, 0.022 and 31.38, 0.02 and 34.65. Three different pellets were obtained and their magnetosome content was extracted to be analyzed in Z-sizer, resulting in an average size of 100 nm. Also aggregates of magnetosomes were found. The findings reported here could serve as a basis for the future scaling up of a biological factory for nanocarriers for multiple research and/or medical applications.

Keywords:
magnetosomes; Magnetospirillum gryphiswaldense; fed-batch culture; magnetotactic bacteria
Degree Program:
Maestría en Ciencias con Especialidad en Biotecnología
Advisors:
Oscar Alejandro Aguilar Jiménez
Committee Member / Sinodal:
Roberto Parra Saldivar; Magdalena de Jesús Rostro Alanís; Yolanda Arlette Santacruz López
Degree Level:
Master in Science with Speciality in Biotechnology
School:
School of Engineering and Sciences
Campus Program:
Campus Monterrey
Discipline:
Ciencias / Sciences
Appears in Collections:
Ciencias Exactas

Full metadata record

DC FieldValue Language
dc.contributor.advisorOscar Alejandro Aguilar Jiménezen
dc.creatorRodríguez Ceja, Jesús Gilbertoen
dc.date.accessioned2017-05-19T22:15:40Z-
dc.date.available2017-05-19T22:15:40Z-
dc.date.issued2017-05-12-
dc.identifier.urihttp://hdl.handle.net/11285/622540-
dc.description.abstract<p><em>In recent years, Magnetospirillum gryphiswaldense has called attention because it is a bacteria capable of producing magnetic nanoparticles (magnetosomes) that can be used in a variety of applications, such as directed therapies for drug delivery. The production of magnetosomes has not reach threshole concentration to ensure feasible for industrial applications. Thus, it is of high importance to increase the magnetosome production. This research evaluated the effect of stirring rate (100, 200, 400 and 600 rpm) and hydrodinamic conditions based on Reynolds number for the grwoth and production of M. gryphiswaldense. Overall, the better results were obtained at 200 rpm, with correspond to higher biomass and oxygen transfer coeficient (K<sub>LA</sub>). Optimal impeler stirring rate was selected to grow bacteria, followed by an anaerobic stage with feeding, to induce the production of magnetosomes. The best magnetosome yield obtained was 18.79 mg/mL*day in a fed-batch culture, 11.78 in continuous culture and 1.4 in batch culture. Their respective specific growth (</em><em>&mu;</em><em>) and generation time (min) were 0.083 and 8.31, 0.022 and 31.38, 0.02 and 34.65. Three different pellets were obtained and their magnetosome content was extracted to be analyzed in Z-sizer, resulting in an average size of 100 nm. Also aggregates of magnetosomes were found. The findings reported here could serve as a basis for the future scaling up of a biological factory for nanocarriers for multiple research and/or medical applications</em>.</p>en
dc.language.isoengen
dc.rightsOpen Accessen
dc.rights.urihttp://creativecommons.org/licenses/by-sa/4.0/*
dc.titleStudies on the growth of magnetospirillum gryphiswaldense under several conditions and its influence on the production of magnetosomesen
dc.typeTesis de Maestríaes
thesis.degree.grantorInstituto Tecnológico y de Estudios Superiores de Monterreyes
thesis.degree.levelMaster in Science with Speciality in Biotechnologyen
dc.contributor.committeememberRoberto Parra Saldivaren
dc.contributor.committeememberMagdalena de Jesús Rostro Alanísen
dc.contributor.committeememberYolanda Arlette Santacruz Lópezen
thesis.degree.disciplineSchool of Engineering and Sciencesen
thesis.degree.nameMaestría en Ciencias con Especialidad en Biotecnologíaen
dc.subject.keywordmagnetosomesen
dc.subject.keywordMagnetospirillum gryphiswaldenseen
dc.subject.keywordfed-batch cultureen
dc.subject.keywordmagnetotactic bacteriaen
thesis.degree.programCampus Monterreyen
dc.subject.disciplineCiencias / Sciencesen
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