Metabolism of 4-chloro-2-nitrophenol in a Gram-positive bacterium, Exiguobacterium sp. PMA

Hdl Handle:
http://hdl.handle.net/11285/613664
Title:
Metabolism of 4-chloro-2-nitrophenol in a Gram-positive bacterium, Exiguobacterium sp. PMA
Authors:
Arora, Pankaj K.; Sharma, Ashutosh; Mehta, Richa; Shenoy, Belle D.; Srivastava, Alok; Singh, Vijay P.
Issue Date:
21/11/2012
Publisher:
Open Access Publisher
Discipline:
Ciencias de la Salud / Health Sciences
Abstract:
Abstract Background Chloronitrophenols (CNPs) are widely used in the synthesis of dyes, drugs and pesticides, and constitute a major group of environmental pollutants. 4-Chloro-2-nitrophenol (4C2NP) is an isomer of CNPs that has been detected in various industrial effluents. A number of physicochemical methods have been used for treatment of wastewater containing 4C2NP. These methods are not as effective as microbial degradation, however. Results A 4C2NP-degrading bacterium, Exiguobacterium sp. PMA, which uses 4C2NP as the sole carbon and energy source was isolated from a chemically-contaminated site in India. Exiguobacterium sp. PMA degraded 4C2NP with the release of stoichiometeric amounts of chloride and ammonium ions. The effects of different substrate concentrations and various inoculum sizes on degradation of 4C2NP were investigated. Exiguobacterium sp. PMA degraded 4C2NP up to a concentration of 0.6 mM. High performance liquid chromatography and gas chromatography–mass spectrometry identified 4-chloro-2-aminophenol (4C2AP) and 2-aminophenol (2AP) as possible metabolites of the 4C2NP degradation pathway. The crude extract of 4C2NP-induced PMA cells contained enzymatic activity for 4C2NP reductase and 4C2AP dehalogenase, suggesting the involvement of these enzymes in the degradation of 4C2NP. Microcosm studies using sterile and non-sterile soils spiked with 4C2NP were carried out to monitor the bioremediation potential of Exiguobacterium sp. PMA. The bioremediation of 4C2NP by Exiguobacterium sp. PMA was faster in non-sterilized soil than sterilized soil. Conclusions Our studies indicate that Exiguobacterium sp. PMA may be useful for the bioremediation of 4C2NP-contaminated sites. This is the first report of (i) the formation of 2AP in the 4C2NP degradation pathway by any bacterium and (iii) the bioremediation of 4C2NP by any bacterium.
Keywords:
4-Chloro-2-nitrophenol; 4-Chloro-2-aminophenol; 2-aminophenol; Biodegradation; Bioremediation; Soil microcosm
Additional Links:
http://microbialcellfactories.biomedcentral.com/articles/10.1186/1475-2859-11-150
Type:
Artículo / Article
Appears in Collections:
Artículos de Revistas

Full metadata record

DC FieldValue Language
dc.contributor.authorArora, Pankaj K.en
dc.contributor.authorSharma, Ashutoshen
dc.contributor.authorMehta, Richaen
dc.contributor.authorShenoy, Belle D.en
dc.contributor.authorSrivastava, Aloken
dc.contributor.authorSingh, Vijay P.en
dc.date.accessioned2016-06-20T03:51:29Z-
dc.date.available2016-06-20T03:51:29Z-
dc.date.issued21/11/2012-
dc.identifier.otherMicrobial Cell Factories. 2012 Nov 21;11(1):150-
dc.identifier.urihttp://dx.doi.org/10.1186/1475-2859-11-150-
dc.identifier.urihttp://hdl.handle.net/11285/613664-
dc.description.abstractAbstract Background Chloronitrophenols (CNPs) are widely used in the synthesis of dyes, drugs and pesticides, and constitute a major group of environmental pollutants. 4-Chloro-2-nitrophenol (4C2NP) is an isomer of CNPs that has been detected in various industrial effluents. A number of physicochemical methods have been used for treatment of wastewater containing 4C2NP. These methods are not as effective as microbial degradation, however. Results A 4C2NP-degrading bacterium, Exiguobacterium sp. PMA, which uses 4C2NP as the sole carbon and energy source was isolated from a chemically-contaminated site in India. Exiguobacterium sp. PMA degraded 4C2NP with the release of stoichiometeric amounts of chloride and ammonium ions. The effects of different substrate concentrations and various inoculum sizes on degradation of 4C2NP were investigated. Exiguobacterium sp. PMA degraded 4C2NP up to a concentration of 0.6 mM. High performance liquid chromatography and gas chromatography–mass spectrometry identified 4-chloro-2-aminophenol (4C2AP) and 2-aminophenol (2AP) as possible metabolites of the 4C2NP degradation pathway. The crude extract of 4C2NP-induced PMA cells contained enzymatic activity for 4C2NP reductase and 4C2AP dehalogenase, suggesting the involvement of these enzymes in the degradation of 4C2NP. Microcosm studies using sterile and non-sterile soils spiked with 4C2NP were carried out to monitor the bioremediation potential of Exiguobacterium sp. PMA. The bioremediation of 4C2NP by Exiguobacterium sp. PMA was faster in non-sterilized soil than sterilized soil. Conclusions Our studies indicate that Exiguobacterium sp. PMA may be useful for the bioremediation of 4C2NP-contaminated sites. This is the first report of (i) the formation of 2AP in the 4C2NP degradation pathway by any bacterium and (iii) the bioremediation of 4C2NP by any bacterium.en
dc.language.isoenen
dc.publisherOpen Access Publisheren
dc.relation.urlhttp://microbialcellfactories.biomedcentral.com/articles/10.1186/1475-2859-11-150en
dc.rightsOpen Access-
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.titleMetabolism of 4-chloro-2-nitrophenol in a Gram-positive bacterium, Exiguobacterium sp. PMAen
dc.typeArtículo / Articleen
dc.contributor.departmentTecnologico de Monterreyen
dc.rights.holderArora et al.; licensee BioMed Central Ltd.-
dc.date.updated2016-06-01T12:18:49Z-
dc.subject.keyword4-Chloro-2-nitrophenolen
dc.subject.keyword4-Chloro-2-aminophenolen
dc.subject.keyword2-aminophenolen
dc.subject.keywordBiodegradationen
dc.subject.keywordBioremediationen
dc.subject.keywordSoil microcosmen
dc.subject.disciplineCiencias de la Salud / Health Sciences-
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