Recovery and Characterization of Proteins from Plants: Aqueous Two-Phase System-Based Bioengineering Strategies

Hdl Handle:
http://hdl.handle.net/11285/572644
Title:
Recovery and Characterization of Proteins from Plants: Aqueous Two-Phase System-Based Bioengineering Strategies
Authors:
Aguilar Jiménez, Oscar A. ( 0000-0002-5352-9579 )
Issue Date:
01/12/2007
Abstract:
The main drawback for general acceptance of plants as economically viable production systems is the lack of efficient initial concentration and separation procedures. In order to facilitate the general acceptance of plants as bioreactors, the establishment of efficient downstream operations is critical. It has been established that with the general knowledge of the molecular properties of contaminant proteins, the selection and design of suitable downstream strategies for recombinant proteins can be improved. The present dissertation addresses the potential use of quantitative 2D electrophoresis (2-DE) coupled with hydrophobic partitioning in aqueous two-phase systems (ATPS) for three-dimensional characterization of proteins from plant extracts. The application of this experimental approach to soybean proteins resulted in molecular characterization of proteins. Molecular weight (MW), isoelectric point (pI) and hydrophobicity were measured simultaneously and demonstrated that this technique can be a valuable tool for predictive design of recovery steps for recombinant proteins from plants. The extension of this experimental approach in alfalfa green tissue extracts containing a model recombinant protein provided additional information on the molecular properties of the main host proteins that will allow the design of pre-fractionation and purification methods to facilitate its recovery from alfalfa extracts. As a result of the application of this three-dimensional characterization technique to soybean and alfalfa protein extracts, more efficient downstream strategies could be designed for recovery of recombinant proteins, facilitating the future adoption of plants as a production system.
Keywords:
Plantas; Disertación
Degree Program:
Programa de Graduados en ingeniería
Advisors:
Marco A. Rito-Palomares
Committee Member / Sinodal:
Charles E. Glastz; Blanca H. Lapizco Encinas; Rocío I. Díaz de la Garza; Manuel I. Zertuche Guerra
Degree Level:
Doctor en ciencias de ingeniería
School:
Escuela de Ingeniería y Arquitectura
Campus Program:
Campus Monterrey
Discipline:
Ingeniería y Ciencias Aplicadas / Engineering & Applied Sciences
Appears in Collections:
Ciencias Exactas

Full metadata record

DC FieldValue Language
dc.contributor.advisorMarco A. Rito-Palomareses
dc.contributor.authorAguilar Jiménez, Oscar A.es
dc.date.accessioned2015-08-17T11:37:15Zen
dc.date.available2015-08-17T11:37:15Zen
dc.date.issued01/12/2007-
dc.identifier.urihttp://hdl.handle.net/11285/572644en
dc.description.abstractThe main drawback for general acceptance of plants as economically viable production systems is the lack of efficient initial concentration and separation procedures. In order to facilitate the general acceptance of plants as bioreactors, the establishment of efficient downstream operations is critical. It has been established that with the general knowledge of the molecular properties of contaminant proteins, the selection and design of suitable downstream strategies for recombinant proteins can be improved. The present dissertation addresses the potential use of quantitative 2D electrophoresis (2-DE) coupled with hydrophobic partitioning in aqueous two-phase systems (ATPS) for three-dimensional characterization of proteins from plant extracts. The application of this experimental approach to soybean proteins resulted in molecular characterization of proteins. Molecular weight (MW), isoelectric point (pI) and hydrophobicity were measured simultaneously and demonstrated that this technique can be a valuable tool for predictive design of recovery steps for recombinant proteins from plants. The extension of this experimental approach in alfalfa green tissue extracts containing a model recombinant protein provided additional information on the molecular properties of the main host proteins that will allow the design of pre-fractionation and purification methods to facilitate its recovery from alfalfa extracts. As a result of the application of this three-dimensional characterization technique to soybean and alfalfa protein extracts, more efficient downstream strategies could be designed for recovery of recombinant proteins, facilitating the future adoption of plants as a production system.en
dc.language.isoenen
dc.rightsOpen Accessen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.titleRecovery and Characterization of Proteins from Plants: Aqueous Two-Phase System-Based Bioengineering Strategiesen
dc.typeTesis de Doctoradoes
thesis.degree.grantorInstituto Tecnológico y de Estudios Superiores de Monterreyes
thesis.degree.levelDoctor en ciencias de ingenieríaes
dc.contributor.committeememberCharles E. Glastzes
dc.contributor.committeememberBlanca H. Lapizco Encinases
dc.contributor.committeememberRocío I. Díaz de la Garzaes
dc.contributor.committeememberManuel I. Zertuche Guerraes
thesis.degree.disciplineEscuela de Ingeniería y Arquitecturaes
thesis.degree.namePrograma de Graduados en ingenieríaes
dc.subject.keywordPlantases
dc.subject.keywordDisertaciónes
thesis.degree.programCampus Monterreyes
dc.subject.disciplineIngeniería y Ciencias Aplicadas / Engineering & Applied Scienceses
All Items in REPOSITORIO DEL TECNOLOGICO DE MONTERREY are protected by copyright, with all rights reserved, unless otherwise indicated.