Analysis of the Dynamics of Neurosecretory Vesicles by Optical Tweezers and Image Processing

Hdl Handle:
http://hdl.handle.net/11285/572489
Title:
Analysis of the Dynamics of Neurosecretory Vesicles by Optical Tweezers and Image Processing
Authors:
Alvarez Elizondo, Martha B.
Issue Date:
01/12/2008
Abstract:
We present the analysis of chromaffin vesicles' dynamics during exocytosis. Optical tweezers combined with fluorescence for noninvasive microviscometry in cells and confocal imaging for trajectory analysis were used for this study. By the use of optical tweezers and fluorescent we applied the oscillation method propose by Fischer et al for measuring the intracellular vis- coelastic properties in cells. A sinusoidally moving optical trap was used to drive intracellular optical trapped vesicles present in chromaffin cells in order to obtain local information about the viscoelastic liquid surrounding the vesicles. For probing this technique measurement were performed on water, glycerol and PEO, then in cells.
Keywords:
Procesadores; Imagen; Dinámicos
Degree Program:
Programa de Graduados en Mecatrónica e Información Tecnológicas
Advisors:
Dr. Rodolfo Rodríguez y Masegosa
Committee Member / Sinodal:
Dr. Julio C. Gutiérrez Vega. Dr. Halina Rubinsztein-Dunlop
Degree Level:
Doctor en Filosofía
School:
Escuela de Graduados en Electrónica, Computación, Información y Comunicación
Campus Program:
Campus Monterrey
Discipline:
Ingeniería y Ciencias Aplicadas / Engineering & Applied Sciences
Appears in Collections:
Ciencias Exactas

Full metadata record

DC FieldValue Language
dc.contributor.advisorDr. Rodolfo Rodríguez y Masegosaes
dc.contributor.authorAlvarez Elizondo, Martha B.es
dc.date.accessioned2015-08-17T11:33:33Zen
dc.date.available2015-08-17T11:33:33Zen
dc.date.issued01/12/2008-
dc.identifier.urihttp://hdl.handle.net/11285/572489en
dc.description.abstractWe present the analysis of chromaffin vesicles' dynamics during exocytosis. Optical tweezers combined with fluorescence for noninvasive microviscometry in cells and confocal imaging for trajectory analysis were used for this study. By the use of optical tweezers and fluorescent we applied the oscillation method propose by Fischer et al for measuring the intracellular vis- coelastic properties in cells. A sinusoidally moving optical trap was used to drive intracellular optical trapped vesicles present in chromaffin cells in order to obtain local information about the viscoelastic liquid surrounding the vesicles. For probing this technique measurement were performed on water, glycerol and PEO, then in cells.en
dc.language.isoenen
dc.rightsOpen Accessen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.titleAnalysis of the Dynamics of Neurosecretory Vesicles by Optical Tweezers and Image Processingen
dc.typeTesis de Doctoradoes
thesis.degree.grantorInstituto Tecnológico y de Estudios Superiores de Monterreyes
thesis.degree.levelDoctor en Filosofíaes
dc.contributor.committeememberDr. Julio C. Gutiérrez Vega. Dr. Halina Rubinsztein-Dunlopes
thesis.degree.disciplineEscuela de Graduados en Electrónica, Computación, Información y Comunicaciónes
thesis.degree.namePrograma de Graduados en Mecatrónica e Información Tecnológicases
dc.subject.keywordProcesadoreses
dc.subject.keywordImagenes
dc.subject.keywordDinámicoses
thesis.degree.programCampus Monterreyes
dc.subject.disciplineIngeniería y Ciencias Aplicadas / Engineering & Applied Scienceses
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