• English
    • español
  • English 
    • English
    • español
  • Login
View Item 
  •   Home
  • Tesis
  • Tesis Doctorales
  • Ciencias Exactas
  • View Item
  •   Home
  • Tesis
  • Tesis Doctorales
  • Ciencias Exactas
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Browse

All of DSpaceCommunitiesTitlesAuthorsIssue DateSubmit DateDisciplineThis CollectionTitlesAuthorsIssue DateSubmit DateDiscipline

My Account

LoginRegister

About

Carta Autorización de TesisPortal de BibliotecasPortal de InvestigadoresCentros de InvestigaciónTec ResearchEmprendimientoSherpa/RomeoArtículos de RevistasFototecaEditorial DigitalProyecto SENERTesis MEEPreguntas Frecuentes

Statistics

Display statistics

Controlled drug delivery strategies: Advances on the synthesis and molecular dynamics of dendrimers, and optimization of liposomes preparation by Dual Asymmetric Centrifugation

  • CSV
  • RefMan
  • EndNote
  • BibTex
  • RefWorks
Thumbnail
Name:
Thesis Final DBT Diana Sampogna.pdf
Size:
13.27Mb
Format:
PDF
Description:
Ph.D. Thesis
Download
Thumbnail
Name:
Acuerdo Biblioteca.pdf
Size:
1.021Mb
Format:
PDF
Description:
Acuerdo para uso de obra
Download
Authors
Sampogna-Mireles, Diana
Issue Date
2017-12-01
Keywords
Dendrimers
Drug delivery systems
Folic acid
Pristimerin
Selectivity
Molecular Dynamics
Liposomes
Dual Asymmetric Centrifugation
biotechnology
Advisors
Valencia Gallegos, Jesús Ángel
Committee Member / Sinodal
Guitiérrez Uribe, Janet Alejandra
Elizondo Martínez, Perla
Hernández Hernández, José Ascención
Aguilar Jimenéz, Oscar Alejandro
Discipline
Ingeniería y Ciencias Aplicadas / Engineering & Applied Sciences

Metadata
Show full item record
Abstract

In early phase of drug development, New Chemical Entities (NCEs) are used as highly active drugs, with limited availability, and high cost. This study presents two alternatives of drug delivery systems, site-specific dendrimers and liposomes, with potential use as carriers of highly active drugs like pristimerin. Advances in the synthesis of site-specific dendrimers with bis-MPA as branching precursor and three key building blocks are reported, including compounds with folic acid (FA) to provide selectivity to folate receptors of cancer cells, pristimerin as cancer drug model, and fluorescein isothiocyanate (FITC) as fluorescent dye for future evaluation as cancer treatment. Five different dendrimer syntheses were reported, differing on their nucleus (bis-MPA or ethylene glycol) and the linker of folic acid (PEG 3350 and triethylene glycol TEG). Different purification techniques, such as normal-phase and reverse-phase column chromatography, size exclusion chromaography, dialysis and ultrafiltration, were probed according to their chemical characteristics and solubility. Characterization was carried out by FT-IR, MALDITOF-MS and/or NMR. Theoretical evidence generated in this work supports that FA-PEG750 and FA-PEG3350 dendrimers can be applied to selectively carry drugs and interact with cancer cell receptors (FR-α). Another drug delivery carrier and a viable alternative to the use of dendrimers is liposomes. Liposomes are the most mature drug carriers for passive and active targeting commercially available in oncology and other disease treatments. Dual Asymmetric Centrifugation (DAC) is a novel, fast, simple, and reproducible method for liposomal formulation screening, it facilitates liposomes preparation, and favors small diameters (<120 nm) in a small scale, with high drug encapsulation (EE). An optimization of DAC parameters (type and volume of buffer, beads size, centrifugation speed and time) was done to obtain liposomes of 70-80 nm with high encapsulation efficiency (71%). Also, dialysis was the best method for liposomes purification in comparison to Zeba-Spin and Micro-Spin size-exclusion columns, and can be applied to other drug delivery systems, like dendrimers. Over all, site-specific dendrimers demand a meticulous control on their structure during synthesis. Their preparation is time consuming and analytically complex. Meanwhile, theoretical evidence supports their potential to selectively carry drugs and interact with cancer cell receptors. Meanwhile, liposomes are a faster, easier and versatile alternative as drug delivery carriers of highly toxic drugs.

Degree Program
Doctorado en Biotecnología
Degree Level
Doctor in Biotechnology
School
School of Engineering and Sciences
Campus Program
Campus Monterrey
Collections
Ciencias Exactas

entitlement

 
DSpace software copyright © 2002-2019  DuraSpace
Quick Guide | Contact Us | Send Feedback
Aviso de Privacidad | Políticas de Privacidad | Acerca del RITEC | Aviso Legal
Open Repository is a service operated by 
Atmire NV
 

Export search results

The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

By default, clicking on the export buttons will result in a download of the allowed maximum amount of items.

To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.