Protein adsorption through Chitosan–Alginate membranes for potential applications
AuthorsMurguía Flores, Dennise A.
Bonilla Ríos, Jaime
Canales Fiscal, Martha R.
Sánchez Fernández, Antonio
DisciplineCiencias de la Salud / Health Sciences
MetadataShow full item record
AbstractAbstract Background Chitosan and Alginate were used as biopolymers to prepare membranes for protein adsorption. The network requires a cross-linker able to form bridges between polymeric chains. Viscopearl-mini® (VM) was used as a support to synthesize them. Six different types of membranes were prepared using the main compounds of the matrix: VM, Chitosan of low and medium molecular weight, and Alginate. Results Experiments were carried out to analyze the interactions within the matrix and improvements were found against porous cellulose beads. SEM characterization showed dispersion in the compounds. According to TGA, thermal behaviour remains similar for all compounds. Mechanical tests demonstrate the modulus of the composites increases for all samples, with major impact on materials containing VM. The adsorption capacity results showed that with the removal of globular protein, as the adsorbed amount increased, the adsorption percentage of Myoglobin from Horse Heart (MHH) decreased. Molecular electrostatic potential studies of Chitosan–Alginate have been performed by density functional theory (DFT) and ONIOM calculations (Our own N-layered integrated molecular orbital and molecular mechanics) which model large molecules by defining two or three layers within the structure that are treated at different levels of accuracy, at B3LYP/6-31G(d) and PM6/6-31G(d) level of theory, using PCM (polarizable continuum model) solvation model. Conclusions Finally, Viscopearl-mini® acts as a suitable support on the matrix for the synthesis of Chitosan–Alginate membranes instead of cross-linkers usage. Therefore, it suggests that it is a promise material for potential applications, such as: biomedical, wastewater treatment, among others. Graphical abstract Chitosan, Alginate, and Cellulose beads-based membranes for protein adsorption. Special attention was given for preparation, charaterization, adsorption capacity, and molecular electrostatic potential studies calculation. Viscopearl-mini® gives support on the matrix of Chitosan–Alginate membranes instead of cross-linkers usage
TypeArtículo / Article
CollectionsArtículos de Revistas
- Chitosan and alginate biopolymer membranes for remediation of contaminated water with herbicides.
- Authors: Agostini de Moraes M, Cocenza DS, da Cruz Vasconcellos F, Fraceto LF, Beppu MM
- Issue date: 2013 Dec 15
- Effect of surface morphology and charge on the amount and conformation of fibrinogen adsorbed onto alginate/chitosan microcapsules.
- Authors: Xie HG, Zheng JN, Li XX, Liu XD, Zhu J, Wang F, Xie WY, Ma XJ
- Issue date: 2010 Apr 20
- Removal of glyphosate herbicide from water using biopolymer membranes.
- Authors: Carneiro RT, Taketa TB, Gomes Neto RJ, Oliveira JL, Campos EV, de Moraes MA, da Silva CM, Beppu MM, Fraceto LF
- Issue date: 2015 Mar 15
- Preparation of alginate-chitosan hybrid gel beads and adsorption of divalent metal ions.
- Authors: Gotoh T, Matsushima K, Kikuchi K
- Issue date: 2004 Apr
- Biosorption of phenol and o-chlorophenol from aqueous solutions on to chitosan-calcium alginate blended beads.
- Authors: Nadavala SK, Swayampakula K, Boddu VM, Abburi K
- Issue date: 2009 Feb 15