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dc.contributor.advisorDr. Rodolfo Rodríguez y Masegosaes
dc.creatorAlvarez Elizondo, Martha B.en
dc.date.accessioned2015-08-17T11:33:33Zen
dc.date.available2015-08-17T11:33:33Zen
dc.date.issued2008-01-12
dc.identifier.urihttp://hdl.handle.net/11285/572489en
dc.description.abstractWe present the analysis of chromaffin vesicles' dynamics during exocytosis. Optical tweezers combined with fluorescence for noninvasive microviscometry in cells and confocal imaging for trajectory analysis were used for this study. By the use of optical tweezers and fluorescent we applied the oscillation method propose by Fischer et al for measuring the intracellular vis- coelastic properties in cells. A sinusoidally moving optical trap was used to drive intracellular optical trapped vesicles present in chromaffin cells in order to obtain local information about the viscoelastic liquid surrounding the vesicles. For probing this technique measurement were performed on water, glycerol and PEO, then in cells.
dc.languageeng
dc.publisherInstituto Tecnológico y de Estudios Superiores de Monterrey
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0*
dc.titleAnalysis of the Dynamics of Neurosecretory Vesicles by Optical Tweezers and Image Processingen
dc.typeTesis de doctorado
thesis.degree.levelDoctor en Filosofíaes
dc.contributor.committeememberDr. Julio C. Gutiérrez Vega. Dr. Halina Rubinsztein-Dunlopes
thesis.degree.disciplineEscuela de Graduados en Electrónica, Computación, Información y Comunicaciónes
thesis.degree.namePrograma de Graduados en Mecatrónica e Información Tecnológicases
dc.subject.keywordProcesadoreses
dc.subject.keywordImagenes
dc.subject.keywordDinámicoses
thesis.degree.programCampus Monterreyes
dc.subject.disciplineIngeniería y Ciencias Aplicadas / Engineering & Applied Scienceses
refterms.dateFOA2018-03-07T07:16:48Z
refterms.dateFOA2018-03-07T07:16:48Z
html.description.abstractWe present the analysis of chromaffin vesicles' dynamics during exocytosis. Optical tweezers combined with fluorescence for noninvasive microviscometry in cells and confocal imaging for trajectory analysis were used for this study. By the use of optical tweezers and fluorescent we applied the oscillation method propose by Fischer et al for measuring the intracellular vis- coelastic properties in cells. A sinusoidally moving optical trap was used to drive intracellular optical trapped vesicles present in chromaffin cells in order to obtain local information about the viscoelastic liquid surrounding the vesicles. For probing this technique measurement were performed on water, glycerol and PEO, then in cells.


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