• Modelling gene expression profiles related to prostate tumor progression using binary states

      Martinez, Emmanuel; Trevino, Victor (Open Access Publisher, 31/05/2013)
      Abstract Background Cancer is a complex disease commonly characterized by the disrupted activity of several cancer-related genes such as oncogenes and tumor-suppressor genes. Previous studies suggest that the process of tumor progression to malignancy is dynamic and can be traced by changes in gene expression. Despite the enormous efforts made for differential expression detection and biomarker discovery, few methods have been designed to model the gene expression level to tumor stage during malignancy progression. Such models could help us understand the dynamics and simplify or reveal the complexity of tumor progression. Methods We have modeled an on-off state of gene activation per sample then per stage to select gene expression profiles associated to tumor progression. The selection is guided by statistical significance of profiles based on random permutated datasets. Results We show that our method identifies expected profiles corresponding to oncogenes and tumor suppressor genes in a prostate tumor progression dataset. Comparisons with other methods support our findings and indicate that a considerable proportion of significant profiles is not found by other statistical tests commonly used to detect differential expression between tumor stages nor found by other tailored methods. Ontology and pathway analysis concurred with these findings. Conclusions Results suggest that our methodology may be a valuable tool to study tumor malignancy progression, which might reveal novel cancer therapies.
    • MIMO channel capacity using antenna selection and water pouring

      Cuan Cortes, Jose V.; Vargas-Rosales, Cesar; Munoz Rodriguez, David (Springer Open, 30/12/2014)
      Abstract The use of multiple-input multiple-output (MIMO) communication systems has attracted considerable attention due to capacity and performance improvements without increasing the required bandwidth or transmission power. Although MIMO improves the transmission rate and provides reliable communication, these advantages come at a high cost, since multiple radio frequency (RF) chains have to be employed. Therefore, cost-effective implementation of MIMO systems remains as an important challenge. In this sense, antenna selection techniques (AST) can help reduce the high cost of MIMO systems, while retaining most of their benefits; and in order to improve channel capacity, transmission power can be allocated efficiently according to the channel experienced by each antenna element. In this paper, we show the channel capacity improvement of MIMO systems, by combining the use of AST and transmission power allocation through the water-pouring algorithm (WPA) for different antenna configurations. We apply WPA and allocate more power to the channel with better conditions, when the MIMO channel is Rayleigh distributed and noise is Gaussian. By employing these two techniques (AST and WPA), we show that channel capacity is significantly improved, with higher capacity values than those obtained using traditional systems which just spread equal power among all the transmit antennas. We also show that bounds on the capacity can be considered when these improvements are used in a MIMO system.
    • Protein adsorption through Chitosan–Alginate membranes for potential applications

      Murguía Flores, Dennise A.; Bonilla Ríos, Jaime; Canales Fiscal, Martha R.; Sánchez Fernández, Antonio; Tecnologico de Monterrey (Springer Open, 30/04/2016)
      Abstract Background Chitosan and Alginate were used as biopolymers to prepare membranes for protein adsorption. The network requires a cross-linker able to form bridges between polymeric chains. Viscopearl-mini® (VM) was used as a support to synthesize them. Six different types of membranes were prepared using the main compounds of the matrix: VM, Chitosan of low and medium molecular weight, and Alginate. Results Experiments were carried out to analyze the interactions within the matrix and improvements were found against porous cellulose beads. SEM characterization showed dispersion in the compounds. According to TGA, thermal behaviour remains similar for all compounds. Mechanical tests demonstrate the modulus of the composites increases for all samples, with major impact on materials containing VM. The adsorption capacity results showed that with the removal of globular protein, as the adsorbed amount increased, the adsorption percentage of Myoglobin from Horse Heart (MHH) decreased. Molecular electrostatic potential studies of Chitosan–Alginate have been performed by density functional theory (DFT) and ONIOM calculations (Our own N-layered integrated molecular orbital and molecular mechanics) which model large molecules by defining two or three layers within the structure that are treated at different levels of accuracy, at B3LYP/6-31G(d) and PM6/6-31G(d) level of theory, using PCM (polarizable continuum model) solvation model. Conclusions Finally, Viscopearl-mini® acts as a suitable support on the matrix for the synthesis of Chitosan–Alginate membranes instead of cross-linkers usage. Therefore, it suggests that it is a promise material for potential applications, such as: biomedical, wastewater treatment, among others. Graphical abstract Chitosan, Alginate, and Cellulose beads-based membranes for protein adsorption. Special attention was given for preparation, charaterization, adsorption capacity, and molecular electrostatic potential studies calculation. Viscopearl-mini® gives support on the matrix of Chitosan–Alginate membranes instead of cross-linkers usage
    • Integration and comparison of different genomic data for outcome prediction in cancer

      Gomez Rueda, Hugo; Martínez Ledesma, Emmanuel; Martínez Torteya, Antonio; Palacios Corona, Rebeca; Treviño, Victor; Tecnologico de Monterrey (Open Access Publisher, 29/10/2005)
      In cancer, large-scale technologies such as next-generation sequencing and microarrays have produced a wide number of genomic features such as DNA copy number alterations (CNA), mRNA expression (EXPR), microRNA expression (MIRNA), and DNA somatic mutations (MUT), among others. Several analyses of a specific type of these genomic data have generated many prognostic biomarkers in cancer. However, it is uncertain which of these data is more powerful and whether the best data-type is cancer-type dependent. Therefore, our purpose is to characterize the prognostic power of models obtained from different genomic data types, cancer types, and algorithms. For this, we compared the prognostic power using the concordance and prognostic index of models obtained from EXPR, MIRNA, CNA, MUT data and their integration for ovarian serous cystadenocarcinoma (OV), multiform glioblastoma (GBM), lung adenocarcinoma (LUAD), and breast cancer (BRCA) datasets from The Cancer Genome Atlas repository. We used three different algorithms for prognostic model selection based on constrained particle swarm optimization (CPSO), network feature selection (NFS), and least absolute shrinkage and selection operator (LASSO).
    • Reference values for amino acids and acylcarnitines in peripheral blood in Quarter horses and American Miniature horses

      Rodríguez-Sánchez, Irám P.; Treviño Alvarado, Víctor M.; del Rosario Torres-Sepúlveda, María; López Saldaña, Liliana A.; Ponce García, Gustavo; López Uriarte, Graciela A.; del Consuelo Ruiz-Herrera, María; Zamora Ávila, Diana E.; Villarreal Pérez, Jesús Z.; Dávalos Aranda, Guillermo; Martínez de Villarreal, Laura E. (Springer Open, 29/09/2015)
      Abstract Background Free amino acids and acylcarnitines circulating in the blood can be used for diagnosis for metabolic illness and imbalances. To date, the normal reference ranges of amino acids and acylcarnitines in horse peripheral blood have not been established. In this study, the concentrations of 12 amino acids and 26 acylcarnitines were determined by tandem mass spectrometry in complete blood from 100 healthy horses (50 Quarter horses (QH) [23 males and 27 females] and 50 American Miniature horses (AMH) [15 males and 35 females]) with no signs of metabolic disease. The means and standard deviations were determined and data statistically analyzed. Findings Concentrations of short, medium, and long chain acylcarnitines were significantly higher in male AMH than in male QH. The concentrations of the amino acids alanine, arginine, glycine, proline (glycogenic), and leucine (ketogenic) were higher in the QH than in the AMH. Female AMH had higher concentrations of propionylcarnitine, leucine, proline, arginine, and ornithine than female QH. Conclusions Normal reference ranges of amino acids and acylcarnitines were established for AMH and QH. Significant differences were found in concentration of these compounds between breeds and gender.
    • Protective mechanisms of medicinal plants targeting hepatic stellate cell activation and extracellular matrix deposition in liver fibrosis

      Duval, Florent; Moreno Cuevas, Jorge E.; González Garza y Barrón, María T.; Rodríguez Montalvo, Carlos; Cruz Vega, Delia E. (Springer Open, 24/12/2014)
      Abstract During chronic liver injury, hepatic stellate cells (HSC) are activated and proliferate, which causes excessive extracellular matrix (ECM) deposition, leading to scar formation and fibrosis. Medicinal plants are gaining popularity as antifibrotic agents, and are often safe, cost-effective, and versatile. This review aims to describe the protective role and mechanisms of medicinal plants in the inhibition of HSC activation and ECM deposition during the pathogenesis of liver fibrosis. A systematic literature review on the anti-fibrotic mechanisms of hepatoprotective plants was performed in PubMed, which yielded articles about twelve relevant plants. Many of these plants act via disruption of the transforming growth factor beta 1 signaling pathway, possibly through reduction in oxidative stress. This reduction could explain the inhibition of HSC activation and reduction in ECM deposition. Medicinal plants could be a source of anti-liver fibrosis compounds.
    • Comparative genomics of bacterial and plant folate synthesis and salvage: predictions and validations

      de Crécy Lagard, Valérie; El Yacoubi, Basma; de la Garza, Rocío D; Noiriel, Alexandre; Hanson, Andrew D; Tecnologico de Monterrey (Open Access Publisher, 23/07/2007)
      Abstract Background Folate synthesis and salvage pathways are relatively well known from classical biochemistry and genetics but they have not been subjected to comparative genomic analysis. The availability of genome sequences from hundreds of diverse bacteria, and from Arabidopsis thaliana, enabled such an analysis using the SEED database and its tools. This study reports the results of the analysis and integrates them with new and existing experimental data. Results Based on sequence similarity and the clustering, fusion, and phylogenetic distribution of genes, several functional predictions emerged from this analysis. For bacteria, these included the existence of novel GTP cyclohydrolase I and folylpolyglutamate synthase gene families, and of a trifunctional p-aminobenzoate synthesis gene. For plants and bacteria, the predictions comprised the identities of a 'missing' folate synthesis gene (folQ) and of a folate transporter, and the absence from plants of a folate salvage enzyme. Genetic and biochemical tests bore out these predictions. Conclusion For bacteria, these results demonstrate that much can be learnt from comparative genomics, even for well-explored primary metabolic pathways. For plants, the findings particularly illustrate the potential for rapid functional assignment of unknown genes that have prokaryotic homologs, by analyzing which genes are associated with the latter. More generally, our data indicate how combined genomic analysis of both plants and prokaryotes can be more powerful than isolated examination of either group alone.
    • Metabolism of 4-chloro-2-nitrophenol in a Gram-positive bacterium, Exiguobacterium sp. PMA

      Arora, Pankaj K.; Sharma, Ashutosh; Mehta, Richa; Shenoy, Belle D.; Srivastava, Alok; Singh, Vijay P.; Tecnologico de Monterrey (Open Access Publisher, 21/11/2012)
      Abstract Background Chloronitrophenols (CNPs) are widely used in the synthesis of dyes, drugs and pesticides, and constitute a major group of environmental pollutants. 4-Chloro-2-nitrophenol (4C2NP) is an isomer of CNPs that has been detected in various industrial effluents. A number of physicochemical methods have been used for treatment of wastewater containing 4C2NP. These methods are not as effective as microbial degradation, however. Results A 4C2NP-degrading bacterium, Exiguobacterium sp. PMA, which uses 4C2NP as the sole carbon and energy source was isolated from a chemically-contaminated site in India. Exiguobacterium sp. PMA degraded 4C2NP with the release of stoichiometeric amounts of chloride and ammonium ions. The effects of different substrate concentrations and various inoculum sizes on degradation of 4C2NP were investigated. Exiguobacterium sp. PMA degraded 4C2NP up to a concentration of 0.6 mM. High performance liquid chromatography and gas chromatography–mass spectrometry identified 4-chloro-2-aminophenol (4C2AP) and 2-aminophenol (2AP) as possible metabolites of the 4C2NP degradation pathway. The crude extract of 4C2NP-induced PMA cells contained enzymatic activity for 4C2NP reductase and 4C2AP dehalogenase, suggesting the involvement of these enzymes in the degradation of 4C2NP. Microcosm studies using sterile and non-sterile soils spiked with 4C2NP were carried out to monitor the bioremediation potential of Exiguobacterium sp. PMA. The bioremediation of 4C2NP by Exiguobacterium sp. PMA was faster in non-sterilized soil than sterilized soil. Conclusions Our studies indicate that Exiguobacterium sp. PMA may be useful for the bioremediation of 4C2NP-contaminated sites. This is the first report of (i) the formation of 2AP in the 4C2NP degradation pathway by any bacterium and (iii) the bioremediation of 4C2NP by any bacterium.
    • Novel way to investigate evolution of children refractory epilepsy by complexity metrics in massive information

      Zavala Yoe, Ricardo; Ramírez Mendoza, Ricardo A.; Cordero, Luz M.; Tecnologico de Monterrey (SpringerOpen Journal, 21/08/2015)
      Abstract Epilepsy demands a major burden at global levels. Worldwide, about 1% of people suffer epilepsy and 30% of them (0.3%) are anticonvulsants resistant. Among them, some children epilepsies are peculiarly difficult to deal with as Doose syndrome (DS). Doose syndrome is a very complicated type of children cryptogenic refractory epilepsy (CCRE) which is traditionally studied by analysis of complex electrencephalograms (EEG) by neurologists. CCRE are affections which evolve in a course of many years and customarily, questions such as on which year was the kid healthiest (less seizures) and on which region of the brain (channel) the affection has been progressing more negatively are very difficult or even impossible to answer as a result of the quantity of EEG recorded through the patient’s life. These questions can now be answered by the application of entropies to massive information contained in many EEG. CCRE can not always be cured and have not been investigated from a mathematical viewpoint as far as we are concerned. In this work, a set of 80 time series (distributed equally in four yearly recorded EEG) is studied in order to support pediatrician neurologists to understand better the evolution of this syndrome in the long term. Our contribution is to support multichannel long term analysis of CCRE by observing simple entropy plots instead of studying long rolls of traditional EEG graphs. A comparative analysis among aproximate entropy, sample entropy, our versions of multiscale entropy (MSE) and composite multiscale entropy revealed that our refined MSE was the most convenient complexity measure to describe DS. Additionally, a new entropy parameter is proposed and is referred to as bivariate MSE (BMSE). Such BMSE will provide graphical information in much longer term than MSE.
    • Quality of peer feedback in relation to instructional design: A comparative study in energy and sustainability MOOCs

      Elizondo-Garcia, Josemaria; Schunn, Christian; Gallardo Córdova, Katherina Edith; Tecnologico de Monterrey (2019-01-01)
      Peer feedback has become a common practice in MOOCs for its capacity to scale formative assessment and feedback on higher-order abilities. Though many practices for improving peer assessment have been examined, there is a lack of knowledge of how instructional design and platform features affect the quality of peer assessment and the relative frequency of different types of peer feedback comments. This study aimed to improve understanding of the relationship between quality of feedback and peer-feedback’ pedagogical design. Peer feedback instructional design and peer feedback comment data were examined from two MOOCS in a similar domain of personal relevance but with substantially different designs. Country of origin of the feedback provider was also examined to control for cultural/linguistic effects. Differences between the two courses were observed in both the pedagogical designs and in the focus of peer comments, suggesting that peer feedback design is an important guide for the focus of peer feedback comments. Furthermore, the results support the idea that instructional design features, mainly the guide’ structure and focus, determine the type of comments that participants will produce and hence receive.
    • Student engagement as predictor of xMOOC completion: An analysis from five courses on energy sustainability

      Guajardo-Leal, Brenda E.; Valenzuela González, Jaime Ricardo; Scott, John (2019)
      Error, no me deja copiar y pegar
    • Systematic Mapping Study of Academic Engagement in MOOC

      Guajardo-Leal, Brenda Edith; Navarro-Corona, Claudia; Valenzuela González, Jaime Ricardo (2019)
      error, no deja copiar y pegar
    • En búsqueda del entendimiento en una torre de Babel: propuesta de un modelo para la innovación educativa en redes de colaboración

      García Vázquez, Nancy Janett; Guajardo-Leal, Brenda Edith; Valenzuela González, Jaime Ricardo (Ediciones Universidad de Salamanca, 2018-12-31)
      Las redes de colaboración potencialmente permiten el desarrollo de innovaciones que generan cambios de alto impacto. Los miembros de una red de esta índole tienen siempre objetivos en común, pero también se caracterizan por tener una amplia diversidad de perfiles: distintos idiomas, disciplinas, experiencias profesionales y niveles educativos, entre otras cualidades. Esto puede, en ocasiones, dificultar el entendimiento entre ellos cuando se ha de trabajar colaborativamente. Este artículo presenta el caso de estudio de una red de innovación educativa; se describe su propósito, su dinámica de trabajo y una serie de tensiones experimentadas en procesos de colaboración. Las tensiones son retos a superar derivados de un trabajo entre personas con características múltiples; y representan conexiones entre los nodos de una red que deben ser atendidas para evitar posibles rupturas. Las tensiones detectadas se tomaron como oportunidades para crear una propuesta de modelo para la colaboración. Dicho modelo tiene como objetivo facilitar los procesos de comunicación y trabajo entre los integrantes de un grupo, promoviendo una colaboración más eficiente.
    • La posibilidad de creencias religioso-regionales. Una aproximación desde el significado de la religión católica en el Occidente de México

      Coss y León Coss y León, Domingo; Vázquez Parra, José C.; Escuela de Humanidades y Educación (Universidad de Costa Rica, 2018-12)
      Cuando hablamos de creencias religiosas, es usual que hagamos referencia a un sistema ideológico por medio del cual un individuo se relaciona con su Dios. Sin embargo, hay algunos de estos códigos morales que consiguen trascender del terreno plenamente espiritual, influyendo de manera muy considerable en la forma en que los individuos se comportan o deciden en la sociedad. El presente artículo busca exponer la posibilidad de creencias de tipo religioso-regionales, las cuales un individuo de una cierta población o ciudad adopte como parte de su forma de ser y elegir, o incluso, como una característica propia de pertenencia, más allá del sentido exclusivamente religioso.
    • Motivation and knowledge: pre-assessment and post-assessment of MOOC participants from an energy and sustainability project

      Valdivia, Juan Antonio; Ramírez-Montoya, María-Soledad; Valenzuela-González, Jaime-Ricardo; Tecnológico de Monterrey (Athabasca University Press, 2018-09)
      Understanding factors promoting or preventing participants’ completion of a massive open online course (MOOC) is an important research topic, as attrition rates remain high for this environment. Motivation and digital skills have been identified as aspects promoting student engagement in a MOOC, and they are considered necessary for success.  However, evaluation of these factors has often relied on tools for which the psychometric properties have not been explored; this suggests that researchers may be working with potentially inaccurate information for judging participants’ profiles. Through a set of analyses (t-test, exploratory factor analysis, correlation), this study explores the relationship between information collected by administering valid and reliable pre and post instruments to measure traits of MOOC attendees. The findings from this study support previously reported outcomes concerning the strong relationships among motivation, previous knowledge, and perceived satisfaction factors for MOOC completers. Moreover, this study provides evidence of the feasibility of developing valid assessments for evaluation purposes.
    • Innovative Education in MOOC for Sustainability: Learnings and Motivations

      Carrera, Jeimmy; Ramírez-Hernández, Darinka (2018-08-23)
      This research project analyzes the motivation and learning perceived by the participants in four Massive Open Online Courses (MOOCs) that use innovative education strategies in order to train the community in Education for Sustainability. These MOOCs were delivered during 2017 and the study forms part of the subproject “Open, Interdisciplinary and Collaborative Innovation to Train in Energy Sustainability through MOOCs”, which was offered in the portal of the Binational Laboratory for Intelligent Energy Sustainability Management and Technology Training. The method utilized was mixed, with a triangulation design approach according to the convergence model. This method consisted of two phases: the first being quantitative, with an online survey designed by experts in a Likert type scale, and the second being qualitative, in which valuations of the users were collected through diverse instruments such as focus groups and observations. The results obtained demonstrate the advantages of designing MOOCs that make use of innovative tools, in order to engage the students as much as possible, and the collateral impact on the development of digital abilities and skills in addition to the learning acquired with respect to sustainability.
    • Diseño y validación de un test de evaluación del potencial en emprendimiento social

      Portuguez Castro, May; Valenzuela González, Jaime Ricardo; Navarro Corona, Claudia; Tecnológico de Monterrey (Ediciones Complutense, 2018-06-05)
      El emprendedor social se reconoce como una persona que es capaz de determinar oportunidades de negocio, a la vez que soluciona un problema que afecta a otras personas. En la literatura se encuentran pocos estudios que estén enfocados hacia este tipo de emprendedor, ya que generalmente se realizan investigaciones sobre los emprendedores en general, sin reconocer las características de los emprendedores sociales. Este estudio valida un instrumento diseñado para medir el potencial en emprendimiento social que tiene una persona. La finalidad del instrumento es que funcione como una herramienta para quienes realizan labores de incubación de empresas, así como de capacitación para los emprendedores sociales; también puede ser utilizado dentro de organizaciones que manejen programas de responsabilidad social, como las cooperativas, con el fin de que tomen en cuenta las necesidades del emprendedor a la hora de diseñar estrategias de desarrollo para estas personas y sus emprendimientos. Además, para que los mismos emprendedores puedan determinar su potencial en el desarrollo de estas empresas.
    • Desigualdad, microcréditos y desarrollo sostenible: un estudio en la Zona Metropolitana de Guadalajara

      Montalvo Corzo, Raúl Francisco; Vázquez Parra, José Carlos; Amézquita Zamora, Juan Alberto; Loría, Eduardo; Escuela de Humanidades y Educación (Universidad Autónoma del Estado de México, 2018-03-15)
      Hablar de desigualdad nos obliga a ahondar en las diferentes herramientas que buscan reducirla, como es el caso de los microcréditos. Se tiene como objetivo primordial presentar una aproximación al impacto real que tienen los microcréditos en la mejora de las condiciones de vida de un grupo de familias de zonas vulnerables de la Zona Metropolitana de Guadalajara, México. Asimismo, se busca argumentar algunos de los cuestionamientos que se dan sobre los parámetros bajo los que se conceden este tipo de préstamos. Como punto de referencia, se hace el análisis de la información recabada a partir de diferentes propuestas teóricas que se encuentran a favor o en contra de la percepción de los microcréditos como una herramienta ideal para reducir la desigualdad
    • Antimicrobial and antiproliferative activity of essential oil, aqueous and ethanolic extracts of Ocimum micranthum Willd leaves

      Caamal-Herrera, Isabel O; Carrillo-Cocom, Leydi M; Escalante-Réndiz, Diana Y; Aráiz-Hernández, Diana; Azamar-Barrios, José A; Tecnológico de Monterrey (Springer Open, 2018-02-08)
      Abstract Background Ocimum micranthum Willd is a plant used in traditional medicine practiced in the region of the Yucatan peninsula. In particular, it is used for the treatment of cutaneous infections and wound healing, however there are currently no existing scientific studies that support these applications. The aim of the present study was to evaluate the antimicrobial and the in vitro proliferative activity (on healthy mammalian cell lines) of the essential oil and extracts (aqueous and ethanolic) of this plant. Methods The minimal inhibitory concentration (MIC) of essential oil and aqueous and ethanolic extracts of Ocimum micranthum leaves against Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa and Candida albicans was determined using the microdilution technique. The in vitro proliferative activity of human fibroblast (hFB) and Chinese hamster ovary (CHO-K1) cells treated with these extracts was evaluated using the MTT test. The hFB cell line was also evaluated using Trypan Blue assay. Results Candida albicans was more susceptible to the ethanolic extract and the aqueous extract (MIC value of 5 μL/mL and 80 μL/mL respectively). In the case of Staphylococcus aureus, Bacillus subtilis, and Pseudomonas aeruginosa, the MIC of the aqueous and ethanolic extract was 125 μL/mL. The aqueous extract showed a significant (p < 0.05) antiproliferative effect on hFB cells at a concentration of 4%, with cell proliferation percentage values of 73.56% and 20.59% by MTT method and Trypan Blue assay, respectively; the same effect was observed for the ethanolic extract at concentration from 0.06% to 0.25% using MTT method and at a concentration from 0.125% to 0.25% using Trypan Blue assay. In CHO-K1 cells an antiproliferative effect was observed at a concentration of 8% of aqueous extract and from 0.06% to 0.25% of ethanolic extract using the MTT method. Conclusion These assays showed that low concentrations of essential oil and extracts of Ocimum micranthum leaves are sufficient to cause an antiproliferative effect on the hFB cell line but do not produce an antimicrobial effect against the microorganisms evaluated. More studies are necessary to improve understanding of the mechanism of action of the compounds implicated in the bioactivities shown by the crude extracts.
    • Primary explant culture and collagen I substrate enhances corneal endothelial cell morphology

      Zavala, Judith; Montalvo-Parra, María-Dolores; Guerrero-Ramírez, Guillermo-Isaac; Rodríguez-Barrientos, Carlos-Alberto; Treviño, Victor; Valdez-García, Jorge E (2018-01-18)
      Abstract Objectives Corneal endothelial cell (CEC) isolation and harvest aim to produce engineered grafts to solve donor corneal tissue shortage. To yield high amounts of CEC maintaining morphological and molecular characteristics, several isolation and culture conditions are reported. Here, we combined direct explant culture, with three different coating conditions and a two-step media approach to compare confluence efficiency, morphology, and specific molecular markers expression. Data description Confluence was reached after 2 weeks in the three coating conditions (Matrigel, collagen I, and in uncoated plates) using a two-step approach (proliferative medium without pituitary extract, followed by stabilizer basal medium). Na/K-ATPase and GPC4 markers were detected by immunocytochemistry while GPC4, CD200, and TJP1 by RT-PCR in the three CEC coating culture conditions. CEC in proliferative medium showed spindle morphology in the three conditions. Polygonal morphology was seen in CEC cultures using basal medium under uncoated and collagen I coated plates. CEC cultured in Matrigel-coated plates remained with spindle morphology in basal medium.